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Clinical Genomics Laboratory Technologist Resume

Philadelphia, PA

SUMMARY

  • Biotechnologist wif over 10 years experience focusing on DNA technology Responsive and focused maintaining effective working relationships wif groups, and individuals Quick learner and independent decision maker Ability to communicate technical concepts through presentations and written reports

TECHNICAL SKILLS

  • 16s Genotyping
  • HiSeq 2500, MiSeq, NovaSeq, Covaris LE220plus
  • DNA extraction from blood
  • Quantstudio 12k Flex (Sybr green)
  • Illumina Library Preparation
  • Standard PCR, Cloning, Gel Eletrophoresis
  • High complexity testing
  • High volume, fast paced work environemnt
  • Clinical and research lab experience
  • LIMS (FileMaker Pro)

PROFESSIONAL EXPERIENCE

Confidential, Philadelphia, PA

Clinical Genomics Laboratory Technologist

Responsibilities:

  • Library preparation, including capture methods wif single and multiplexed DNA and RNA samples for illumina sequencing platform
  • Troubleshoots and documents data in LIMS for sample management and workflow tracking
  • Operation and troubleshooting of COVARIS LE220plus, TapeStation, Quantstudio, HiSeq 2500, NovaSeq and Miseq
  • Contributes to laboratory support, including training of new clinical staff, validation of new reagents, monitoring of lab supply inventory and general lab maintenance
  • Follows lab SOP’s and CAP guidelines and maintains the confidentiality of health information on all patients.

Confidential Providence, PA

Bench Scientist (Contract Assignment)

Responsibilities:

  • Created indexed Illumina libraries from extracted DNA and RNA supporting research projects
  • Analyzed nucleic acids using Tape Station data and agarose gels
  • Accurately quantified samples via Quantstudio for sequencing
  • Operation and troubleshooting of MiSeq system
  • Provided DNA Sanger Sequencing service to in house laboratories
  • Processed sequencing data via Nautilus LIMS for automated workflow
  • Maintained DNA sanger sequencer, 3730XL and MiSeq

Confidential, Wilmington, DE

Associate Investigator/Sr. Associate Investigator

Responsibilities:

  • Developed bioassay experiments to study plants treated wif chemical treatments
  • Observed, collected, analyzed and presented data from the greenhouse and field studies, leading to and supporting further investigations
  • Initiated 16s & ITS2 typing experiments to identify microbes from contaminated plants
  • Studied the effect of peptide nucleic acid (PNA) primers as PCR blockers to distinguish the microbial 16s gene from the chloroplast 16s gene
  • Liaison for the purchase of plant culture media maintaining supply and budget
  • Managed high throughput aseptic plant micropropagation and research experiment scheduling
  • Evaluated SOP’s and hazard documents, ensuring safety during laboratory procedures
  • Trained new employees in aseptic micropropagation, standardizing lab operations.
  • Interviewed candidates for new associate positions
  • Managed vector quality control and was subject matter expert (SME) for hundreds of DNA transgenes per year, assuring trait purity standards under ISO9000.
  • Supported DNA cloning of transgenes into E.coli and yeast including, extraction and analysis of DNA inserts from colonies to create genetically - modified plants.
  • Selected restriction enzymes for DNA digestion using Vector NTI
  • Prepared solutions, ordered lab supplies and services to maintain lab operations
  • Maintained detailed experimental results in eLNB

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