PROFESSIONAL EXPERIENCE:

Confidential, Saint Paul, MN

Advanced Applications Specialist - Technical Specialist

Responsibilities:

• Provided customer technical support for automated Immunoassay instruments and assays. Troubleshoot software, hardware, assay performance, QC and calibrations.

Confidential, Chesterfield, MO

Automation Engineer - Research Associate

Responsibilities:

• Programed and developed Confidential methods to increase throughput, efficiencies, and reduce costs for Molecular Breeding Technologies.
• Member of the STL Biotechnology ISO 9001 Internal Auditor, QMS Champion
• Participated in Biotech Lean Practitioner .
• Utilized Spotfire Visualization tool for Maintenance Connection for analysis of work orders, technicians, types of repairs and equipment locations.
• Participated on the team implementing Splunk to track equipment processes, down time, error logs, and frequency of equipment failures.
• Experienced in programming various automated platforms including Confidential, Confidential, Confidential, Confidential, Confidential, and other bench top liquid handlers. Successfully developed, validated, and implemented multiple automated assays in 96, and 384 well automated formats. Maintain the instrumentation according to SOP, GLP, and QC requirements.
• Basic understanding of agricultural genetics including DNA extraction, invader assay technology, and data analysis
• Assessed and recommended new automation to meet or exceed current processes, within a stringent timeframe. Purchased approximately 1.5 million capital items within 6 months of hire. Coordinated the installation and site preparation for new automation, including assessing space and workflow concerns.
• Implemented new custom automation for Mega Soybean Chippers with partnering St. Louis colleagues. Support and maintain Mega Soybean Chippers automation, troubleshooting and maintenance.
• Trained multiple colleagues on troubleshooting, programming, error recovery and development of automated assays on multiple automated platforms.

Confidential, River Falls, WI

Laboratory Assistant

Responsibilities:

• Basic knowledge on qualitative trait testing (Cry1AB, Cry1F, Cry3Bb, Cry2Ab, CP4, Cry34) on corn crops using ELISA methods, Confidential, and Confidential automation.
• Experience with planting, harvesting and evaluation of corn and soybean crops, for ELISA and Herbicide (Liberty and Roundup Ready) studies.
• Basic understanding on Standard Seed Testing (SST), for cold/warm seed viability/evaluation/planting.
• Basic understanding on Breeder studies (ELISA, Herbicide Bio-Assay), including planting, and evaluation.

Confidential, La Jolla, CA

Global Research & Development, Scientist

Responsibilities:

• Successfully developed, validated, and implemented multiple in-vitro ADME assays in 24, 96, and 384 well automated formats, including caco-2, porcine bovine microvessel endothiel cells (PBMEC), blood brain barrier transporters (hTAUT1, hSMVT1, hCT1, hCAT1 and hLAT1), liver uptake transporters (OATP-8, OATP-B, OATP-C), kidney transporter (OAT1), intestinal efflux MDCK-MDR1, ELISA and metabolism studies (rat and human microsomes, rat,dog and human hepatocytes).
• Strong background in the development and validation of transporter science including blood-brain barrier, liver, kidney, and intestinal transporters.
• Experienced in cell culture techniques for multiple in-vitro cell lines (caco-2, MDCK-MDR1, PBMEC, OATP-8, OATP-B, OATP-C, hTAUT1, hSMVT1, hCT1, hCAT1, hLAT1, HEK wild type). Experienced in programming various automated platforms including Confidential, Confidential, Confidential and other bench top liquid handlers. Maintained the instrumentation according to SOP, GLP, and QC requirements.
• Basic understanding of biotherapeutics including vaccines, monoclonal antibodies, western blot, immunoassay and flow cytometry.
• Participated on IT related teams developing internal software applications for data analysis, data storage, and data retrieval with 21 CFR 11 compliance.
• Participated on global teams for in-vitro ADME related studies to discuss assay performance, utility, QC guidelines, and data interpretation guidelines.
• Knowledgeable with computational ADME science, SAR, in-silico modeling, and Spotfire visualization tool.
• Prepared IV/PO dose formulations for PK/PD pre-clinical rat and dog in-vivo studies. Performed PK/PD pre-clinical in-vivo dog studies, including IV/PO dosing and intravenous sample collection.
• Experienced with computers including extensive knowledge of Microsoft: Excel, Access, PowerPoint, Word, and Visual Basic. Strong IT background with internal applications including RGate (nominated as RGate Champion), IVAD, GeCA3.0, Screening Services, Pipeline Pilot.
• Trained multiple scientists in cell culture techniques, automation execution (calibrations, trouble shooting), in-vitro ADME data analysis and QC, and internal applications.

Confidential, Saint Paul, MN

Responsibilities:

• Automated, programmed, developed, implemented and validated new assays.
• Maintain operation and processes related to production operation, while trying to maintain cycle times.
• Trained colleagues on programming and troubleshooting, on multiple automated platforms.
• Implemented and partnered with other contractors and colleagues for site installation on two new systems.
• Coordinated and partnered with Confidential Sales, Field Engineers, Project Field Managers in the purchase, design, and installation of our Confidential Integrated system and our dual multichannel Confidential .

Confidential, Middleton, WI

Responsibilities:

• Automated, programmed, developed, implemented and validated new assays.
• Maintain operation and processes related to production operation, while trying to maintain cycle times.
• Trained colleagues on programming and troubleshooting, on multiple automated platforms.
• Implemented and partnered with other contractors and colleagues for site installation on two new systems.
• Coordinated and partnered with Confidential Sales, Field Engineers, Project Field Managers in the purchase, design, and installation of our Confidential Integrated system and our dual multichannel Confidential

Confidential, River Falls, WI

Laboratory Assistant

Responsibilities:

• Basic knowledge on qualitative trait testing (Cry1AB, Cry1F, Cry3Bb, Cry2Ab, CP4, Cry34) on corn crops using ELISA methods, Confidential, and Confidential automation.
• Experience with planting, harvesting and evaluation of corn and soybean crops, for ELISA and Herbicide (Liberty and Roundup Ready) studies.
• Basic understanding on Standard Seed Testing (SST), for cold/warm seed viability/evaluation/planting.

Confidential, La Jolla, CA

Global Research & Development, Scientist

Responsibilities:

• Provided support for ADME related studies to support CNS and Oncology drug discovery projects.
• Automated, programmed, developed, implemented and validated new assays.
• Supervised ADME assay execution, including scheduling, cycle-times, and QC.
• Participated on IT related teams to develop new in-house software to improve data handling.

Confidential, Indianapolis, IN

Senior Laboratory Technician

Responsibilities:

• Performed automated caco-2 studies, and HPLC method development, according to GMP and SOP.
• Programmed using Visual Basic and Microsoft Access for data handling and storage.

Confidential, Indianapolis, IN

Research Technician

Responsibilities:

• Analyzed various isotopes (C13, O18, and H2) using IRMS instrumentation and performed combustion experiments with cholesterol and triglycerides to study energy expenditure and lipid metabolism.

Confidential, Lake Geneva, WI

Polymer Chemist

Responsibilities:

• Practical knowledge in the research and development of polymer-based rubber compounds.
• Evaluated alternative raw chemical ingredients as potential replacements to reduce material costs and improve material processing.
• Participated on cross-functional teams to reduce product to reduce product defects, and manufacturing loss. Performed failure analysis on part failures.
• Conducted physical testing on polymer-based rubber compounds using rheometers, tensiometers, mooney and gehman instrumentation, to support production.
• Monitored and reviewed QC for production approval of compound batches for molding readiness.
• Created, revised SOP’s and DOP’s for QS 9000 .