Bench Scientist Resume Philadelphia, PA - Hire IT People

SUMMARY

Biotechnologist with over 10 years experience focusing on DNA technology
Responsive and focused maintaining effective working relationships with groups, and individuals
Quick learner and independent decision maker
Ability to communicate technical concepts through presentations and written reports

TECHNICAL SKILLS

PROFESSIONAL EXPERIENCE

Confidential, Philadelphia, PA

Responsibilities:

Library preparation, including capture methods with single and multiplexed DNA and RNA samples for illumina sequencing platform
Troubleshoots and documents data in LIMS for sample management and workflow tracking
Operation and troubleshooting of COVARIS LE220plus, TapeStation, Quantstudio, HiSeq 2500, NovaSeq and Miseq
Contributes to laboratory support, including of new clinical staff, validation of new reagents, monitoring of lab supply inventory and general lab maintenance
Follows lab SOP’s and CAP guidelines and maintains the confidentiality of health information on all Confidential .

Confidential, Upper Providence, PA

Bench Scientist

Responsibilities:

Created indexed Illumina libraries from extracted DNA and RNA supporting research projects
Analyzed nucleic acids using Tape Station data and agarose gels
Accurately quantified samples via Quantstudio for sequencing
Operation and troubleshooting of MiSeq system
Provided DNA Sanger Sequencing service to in house laboratories
Processed sequencing data via Nautilus LIMS for automated workflow
Maintained DNA sanger sequencer, 3730XL and MiSeq

Confidential, Wilmington, DE

Associate Investigator

Responsibilities:

Developed bioassay experiments to study plants treated with chemical treatments
Observed, collected, analyzed and presented data from the greenhouse and field studies, leading to and supporting further investigations
Initiated 16s & ITS2 typing experiments to identify microbes from contaminated plants
Studied the effect of peptide nucleic acid (PNA) primers as PCR blockers to distinguish the microbial 16s gene from the chloroplast 16s gene
Liaison for the purchase of plant culture media maintaining supply and budget
Managed high throughput aseptic plant micropropagation and research experiment scheduling
Evaluated SOP’s and hazard documents, ensuring safety during laboratory procedures
Trained new employees in aseptic micropropagation, standardizing lab operations.
Interviewed candidates for new associate positions
Managed vector quality control and was subject matter expert (SME) for hundreds of DNA transgenes per year, assuring trait purity standards under ISO9000.
Supported DNA cloning of transgenes into E.coli and yeast including, extraction and analysis of DNA inserts from colonies to create genetically - modified plants.
Selected restriction enzymes for DNA digestion using Vector NTI
Prepared solutions, ordered lab supplies and services to maintain lab operations
Maintained detailed experimental results in eLNB