Assistant Specialist Resume Riverside - Hire IT People

SUMMARY:

Reviving, maintaining and splitting cells
Transient transfection of mammalian cells
Localization of proteins by confocal microscopy
Fluorescence Resonance Energy Transfer (FRET)
Solvent extraction of plant metabolites
Solid phase micro - extraction (SPME) of volatile from plants
Metabolic analysis with Gas Chromatography-Mass Spectrometry (GC-MS)
DNA and RNA isolation
PCR, real time PCR
Molecular cloning
DNA sequencing
RNA sequencing
Southern blot and Northern blot
Construction of cDNA expression library
Yeast one-hybrid
Electrophoretic mobility shift assay (EMSA)
Protein isolation and characterization from transgenic plants
Western blotting
Recombinant protein expression and large-scale purification through AKTA system
Yeast two-hybrid
Calmodulin-binding assay
Co-immunoprecipitation
Agrobacterium-mediated transformation in Tobacco plants and rice callus
Tissue culture of rice callus, tobacco leaf
Construction of plant expression binary vector
Construction of activation tagging mutant library
Agrobacterium-mediated floral dip transformation in Arabidopsis and Camelina
High-throughput screening of Arabidopsis mutants under salt, drought and cold stresses
Pathogen inoculation and plant disease resistance assay

RESEARCH EXPERIENCE:

Assistant Specialist

Confidential, Riverside

Responsibilities:

Dynamic study of DNMT1/UHRF1 in living mammalian cells through fluorescence resonance energy transfer (FRET).
Large-scale purification of different proteins through AKTA system.

Postdoctoral Research Associate

Confidential

Responsibilities:

Understanding the molecular mechanism of and improve large fragment transfer through Agrobacterium-mediated monocot (rice) transformation.
Identifying new components in the Nicotine biosynthetic pathway.

Postdoctoral Research Associate

Confidential

Responsibilities:

Metabolic programming and pathway engineering of terpenes in Camelina sativa.
Manipulation of monoterpene biosynthetic pathways by ectopically expressing an insect geranyl pyrophosphate synthase in Camelina sativa.

Lab Assistant

Confidential, Chapel Hill

Responsibilities:

Graduate Research Assistant

Confidential

Responsibilities:

Investigating the function of calcium/calmodulin-binding transcription factor family AtSR in biotic stress defense.
Investigating the function of calcium/calmodulin-binding trihelix transcription factor AtGT2L in abiotic stress responses.